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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
12/12/2012 |
Data da última atualização: |
09/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VAZ, F. A. S.; SILVA, P. A. DA; PASSOS, L. P.; HELLER, M.; MICKE, G. A.; COSTA, A. C. O.; OLIVEIRA, M. A. L. DE. |
Afiliação: |
FERNANDO ANTONIO SIMAS VAZ, UFJF; PATRÍCIA APARECIDA DA SILVA, UFJF; LEONIDAS PAIXAO PASSOS, CNPGL; MELINA HELLER, UFSC; GUSTAVO AMADEU MICKE, UFSC; ANA CAROLINA OLIVEIRA DA COSTA, UFSC; MARCONI AUGUSTO LEAL DE OLIVEIRA, UFJF. |
Título: |
Optimisation of a capillary zone electrophoresis methodology for simultaneous analysis of organic aliphatic acids in extractis of Brachiaria brizantha. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Phytochemical Analysis, v. 23, n. 6, p. 569-575, 2012. |
DOI: |
https://doi.org/10.1002/pca.2355 |
Idioma: |
Inglês |
Conteúdo: |
Introduction: Aluminum toxicity is commonly verified in acidic soils, and poses a severe limitation to plant growth and development. Therefore, Al complexation by the root system mucilage, Al complexation by organic compounds that are exuded by the roots and internal metabolic processes must be monitored by organic acids (OA), since they play a central role in these aluminum tolerance mechanisms. Objective: To optimise a capillary zone electrophoresis method able to perform simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric and aspartic acid in plant extract solutions. Methodology: Method optimisation was achieved by a chemometric approach through experimental designs. The optimal condition found was: 20 mmol/L phthalic acid buffer; 0.8 mmol/L cetyltrimethyl-ammonium bromide; pH 3.4 adjusted with tris(hydroxymethyl)aminomethane (around 16 mmol/L); -15 kV of voltage; 25 °C of cartridge temperature; indirect ultraviolet detection at 240 nm; and 25 mbar injection for 2 s, within an analysis time of 4 min. Results: As a repeatability test of the optimal condition, 30 replicates were carried out with the same working electrolyte, where the relative standard deviation of each peak ranged from 0.081 to 0.36% (for migration time) and from 2.4 to 4.6% (for peak area). Conclusion: The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria brizantha, demonstrating its usefulness to study aluminum tolerance. MenosIntroduction: Aluminum toxicity is commonly verified in acidic soils, and poses a severe limitation to plant growth and development. Therefore, Al complexation by the root system mucilage, Al complexation by organic compounds that are exuded by the roots and internal metabolic processes must be monitored by organic acids (OA), since they play a central role in these aluminum tolerance mechanisms. Objective: To optimise a capillary zone electrophoresis method able to perform simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric and aspartic acid in plant extract solutions. Methodology: Method optimisation was achieved by a chemometric approach through experimental designs. The optimal condition found was: 20 mmol/L phthalic acid buffer; 0.8 mmol/L cetyltrimethyl-ammonium bromide; pH 3.4 adjusted with tris(hydroxymethyl)aminomethane (around 16 mmol/L); -15 kV of voltage; 25 °C of cartridge temperature; indirect ultraviolet detection at 240 nm; and 25 mbar injection for 2 s, within an analysis time of 4 min. Results: As a repeatability test of the optimal condition, 30 replicates were carried out with the same working electrolyte, where the relative standard deviation of each peak ranged from 0.081 to 0.36% (for migration time) and from 2.4 to 4.6% (for peak area). Conclusion: The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria bri... Mostrar Tudo |
Palavras-Chave: |
Acidos organicos; Design fatorial; Tolerancia a aluminio; Zona capilar. |
Thesagro: |
Brachiaria Brizantha; Eletroforese. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02457naa a2200277 a 4500 001 1942177 005 2024-02-09 008 2012 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1002/pca.2355$2DOI 100 1 $aVAZ, F. A. S. 245 $aOptimisation of a capillary zone electrophoresis methodology for simultaneous analysis of organic aliphatic acids in extractis of Brachiaria brizantha.$h[electronic resource] 260 $c2012 520 $aIntroduction: Aluminum toxicity is commonly verified in acidic soils, and poses a severe limitation to plant growth and development. Therefore, Al complexation by the root system mucilage, Al complexation by organic compounds that are exuded by the roots and internal metabolic processes must be monitored by organic acids (OA), since they play a central role in these aluminum tolerance mechanisms. Objective: To optimise a capillary zone electrophoresis method able to perform simultaneous separation of acetic, citric, formic, lactic, malic, oxalic, pyruvic, succinic, tartaric and aspartic acid in plant extract solutions. Methodology: Method optimisation was achieved by a chemometric approach through experimental designs. The optimal condition found was: 20 mmol/L phthalic acid buffer; 0.8 mmol/L cetyltrimethyl-ammonium bromide; pH 3.4 adjusted with tris(hydroxymethyl)aminomethane (around 16 mmol/L); -15 kV of voltage; 25 °C of cartridge temperature; indirect ultraviolet detection at 240 nm; and 25 mbar injection for 2 s, within an analysis time of 4 min. Results: As a repeatability test of the optimal condition, 30 replicates were carried out with the same working electrolyte, where the relative standard deviation of each peak ranged from 0.081 to 0.36% (for migration time) and from 2.4 to 4.6% (for peak area). Conclusion: The methodology was successfully applied to simultaneously determine citric, malic and aspartic acid in roots and leaves extract solutions of Brachiaria brizantha, demonstrating its usefulness to study aluminum tolerance. 650 $aBrachiaria Brizantha 650 $aEletroforese 653 $aAcidos organicos 653 $aDesign fatorial 653 $aTolerancia a aluminio 653 $aZona capilar 700 1 $aSILVA, P. A. DA 700 1 $aPASSOS, L. P. 700 1 $aHELLER, M. 700 1 $aMICKE, G. A. 700 1 $aCOSTA, A. C. O. 700 1 $aOLIVEIRA, M. A. L. DE 773 $tPhytochemical Analysis$gv. 23, n. 6, p. 569-575, 2012.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
06/05/2015 |
Data da última atualização: |
06/05/2015 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
VIDOTTI, M. S.; BORBA, T. C. O.; MELLO, R. N.; ABREU, A. G.; COLOMBARI FILHO, J. M. |
Afiliação: |
MIRIAM SUZANE VIDOTTI, mestranda UFG; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; ALUANA GONCALVES DE ABREU, CNPAF; JOSE MANOEL COLOMBARI FILHO, CNPAF. |
Título: |
Validação do QTL PUP1 para tolerância à deficiência de fósforo em arroz de terras altas. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: CONFERENCIA INTERNACIONAL DO ARROZ PARA AMÉRICA LATINA E CARIBE, 12., 2015, Porto Alegre. Horizonte para a competitividade: anais. Porto Alegre: IRGA, 2015. |
Idioma: |
Português |
Conteúdo: |
O objetivo deste estudo é validar em progênies S0:2 da população CNA9/3/1 (de seleção recorrente) a presença do QTL Phosphorus uptake 1 (Pup1). Nesse sentido, foram avaliados sete marcadores moleculares codominantes e nove dominantes, específicos para esse QTL. |
Thesagro: |
Arroz; Deficiência do solo; Fósforo; Oryza sativa. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/123335/1/p85.pdf
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Marc: |
LEADER 00992nam a2200205 a 4500 001 2014933 005 2015-05-06 008 2015 bl uuuu u00u1 u #d 100 1 $aVIDOTTI, M. S. 245 $aValidação do QTL PUP1 para tolerância à deficiência de fósforo em arroz de terras altas.$h[electronic resource] 260 $aIn: CONFERENCIA INTERNACIONAL DO ARROZ PARA AMÉRICA LATINA E CARIBE, 12., 2015, Porto Alegre. Horizonte para a competitividade: anais. Porto Alegre: IRGA$c2015 520 $aO objetivo deste estudo é validar em progênies S0:2 da população CNA9/3/1 (de seleção recorrente) a presença do QTL Phosphorus uptake 1 (Pup1). Nesse sentido, foram avaliados sete marcadores moleculares codominantes e nove dominantes, específicos para esse QTL. 650 $aArroz 650 $aDeficiência do solo 650 $aFósforo 650 $aOryza sativa 700 1 $aBORBA, T. C. O. 700 1 $aMELLO, R. N. 700 1 $aABREU, A. G. 700 1 $aCOLOMBARI FILHO, J. M.
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